MEDIMEAS MRM-RM MANUAL ROTARY MICROTOME
Histopathology
Microtomes are an essential part of any Histopathology Lab. The purpose of the blog is to educate readers on latest Microtomes available, the issues faced by Histotechnologists in Microtomy and their remedies. We invite histotechnologists to share their experiences in Microtomy.
Sunday 25 September 2011
Monday 6 June 2011
Microtome Manual
Please write to us at contact@medimeas.com to get a free copy of Microtome Brochure and Manual.
Friday 27 May 2011
Difference between a Microtome and a Cryostat
A Microtome is used to cut very thin sections at room temperature, on the other hand a Cryostat is used to cut frozen sections at sub zero temperatures (generally -30 deg C).
A cryostat is used in situations where rapid analysis of tissues is required. The water rich tissue is frozen on a quick freezing shelf inside the cryostat, this makes it very hard and it is then ready to be cut into thin sections in a microtome, also placed inside the cryostat chamber.
On the other hand to be cut in a simple microtome, the tissue needs to be first dehydrated and fixed in paraffin before it can be sectioned. It is a long procedure compared to quick sectioning in a cryostat.
The quality of sections cut in a cryostat is inferior compared to those cut in a microtome because dehydrated and paraffin embedded tissues give better sections but when it comes to quick sectioning, Cryostat is the choice.
A cryostat is used in situations where rapid analysis of tissues is required. The water rich tissue is frozen on a quick freezing shelf inside the cryostat, this makes it very hard and it is then ready to be cut into thin sections in a microtome, also placed inside the cryostat chamber.
On the other hand to be cut in a simple microtome, the tissue needs to be first dehydrated and fixed in paraffin before it can be sectioned. It is a long procedure compared to quick sectioning in a cryostat.
The quality of sections cut in a cryostat is inferior compared to those cut in a microtome because dehydrated and paraffin embedded tissues give better sections but when it comes to quick sectioning, Cryostat is the choice.
Saturday 21 May 2011
Choosing Among Manual, Semi Automatic and Fully Automatic Microtome
The main factor in your Microtome buying decision would be the throughput required.
For Low throughput : Manual
For Medium throughput : Semi Automatic
For High throughput : Fully Automatic
Thursday 19 May 2011
Difference between a Manual, Semi Automatic and Fully Automatic Microtome
In a manual microtome, specimen feed and the rotary motion (cutting stroke) is done by hand.
In a semi automatic microtome, specimen feed is motorized but the rotary motion is still done by hand.
On the other hand in a fully automatic microtome, both the specimen feed and the rotary motion is motorized.
In a semi automatic microtome, specimen feed is motorized but the rotary motion is still done by hand.
On the other hand in a fully automatic microtome, both the specimen feed and the rotary motion is motorized.
Wednesday 11 May 2011
Features of a Manual Cryostat Microtome
The following features are basic and essential in a manual cryostat microtome
1. Thermostatically controlled chamber temperature, preferably as low as -40 deg C
2. Frost Free glass for clear visibility during sectioning
3. Automatic Retraction for uniform sections.
4. Cooling down time should be less than 60 mins.
5. Auto Defrosting
6. Built in Anti Glare Illumination
7. Preferably SS chamber which is easy to clean.
8. Built in Drainage System
9. Should have castors for easy mobility
10. Digital Display of chamber temperature
11. At least 10 freezing stations.
12. Universal Knife Holder
13. Handwheel lockable in any position
1. Thermostatically controlled chamber temperature, preferably as low as -40 deg C
2. Frost Free glass for clear visibility during sectioning
3. Automatic Retraction for uniform sections.
4. Cooling down time should be less than 60 mins.
5. Auto Defrosting
6. Built in Anti Glare Illumination
7. Preferably SS chamber which is easy to clean.
8. Built in Drainage System
9. Should have castors for easy mobility
10. Digital Display of chamber temperature
11. At least 10 freezing stations.
12. Universal Knife Holder
13. Handwheel lockable in any position
Friday 29 April 2011
Features of A Semi Automatic Microtome
What features to look for when buying a semi automatic microtome for your histology lab?
A Semi Automatic Microtome is an enhancement over manual sectioning, as it incorporates high precision motorized specimen feed for efficient operations and consistently uniform sections.
The following features are a must in a Semi Automatic Microtome:
1. Section and Trim Modes
2. Fast Forward Function
3. Fast Backward Function
4. Section/Trim thickness setting function.
5. LCD that displays section thickness and section counts
6. Feather touch keypad
7. A Universal knife holder
8. Interchangeability between universal casette clamp and standard specimen clamp
9. A knife guard
10. Lockable Hand Wheel
11. Automatic Retraction
12. Anti Roll Plate
13. LED visual limit indicators/ alarm system
14. Section waste tray for tissue debris
A Semi Automatic Microtome is an enhancement over manual sectioning, as it incorporates high precision motorized specimen feed for efficient operations and consistently uniform sections.
The following features are a must in a Semi Automatic Microtome:
1. Section and Trim Modes
2. Fast Forward Function
3. Fast Backward Function
4. Section/Trim thickness setting function.
5. LCD that displays section thickness and section counts
6. Feather touch keypad
7. A Universal knife holder
8. Interchangeability between universal casette clamp and standard specimen clamp
9. A knife guard
10. Lockable Hand Wheel
11. Automatic Retraction
12. Anti Roll Plate
13. LED visual limit indicators/ alarm system
14. Section waste tray for tissue debris
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